生命科学与技术学院
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| 导师代码： |
10477
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| 导师姓名： |
汤丽霞
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| 性 别： |
女 |
| 特 称： |
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| 职 称： |
教授
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| 学 位： |
哲学博士学位
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| 属 性： |
专职 |
| 电子邮件： |
l.x.tang@163.com
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| 学术经历：
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09/1987-07/1992获得北京师范大学生物系生物化学专业学士学位；07/1992-09/1996任中国科学院生物物理研究所细胞室实习研究员；09/1996-03/1999任中国科学院生物物理研究所细胞室助理研究员；03/1999-10/2004获得荷兰格罗宁根大学生物化学系博士学位；10/2004-06/2006在荷兰格罗宁根大学生物化学系做博士后研究；07/2006-07/2014任电子科技大学生命科学与技术学院副教授; 07/2014-至今任电子科技大学生命科学与技术学院教授。
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| 个人简介：
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主要研究方向为：1.蛋白质工程；2.酶动力学；3．分子定向进化；4.生物催化。目前主要从事卤醇脱卤酶的研究工作，卤醇脱卤酶是一类通过分子内亲核取代机制催化邻卤醇转化为环氧化物的脱卤酶，可以高效高选择地催化环氧化物和邻卤醇之间的转化，可以用来合成一类具有光学纯的高价值中间体，环氧化物及β-取代醇等，因此，卤醇脱卤酶在手性药物及精细化工合成领域具有十分重要的应用前景，从菌种选育、酶性质分析、酶的分子克隆，到酶高效表达以及手性药物中间体合成都进行了大量的研究工作。现主持研究包括自然科学基金，科技部中克政府间合作项目等6项，先后在国内外期刊正式发表论文近50篇，其中SCI收录的30余篇。
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| 科研项目：
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国家自然基金面上项目：项目名称：基于多时间尺度探究卤醇脱卤酶催化功能的结构动态性基础及作用机制（编号：21673034）；国家自然基金：项目名称：探究卤醇脱卤酶催化合成手性β-取代醇相关机制及其改造研究（编号：21342005）；国家科技重大专项项目子课题项目名称：制药工业废水生物处理关键技术研究（编号ZX0720300）；国家自然科学基金面上项目：项目名称：基于 CASTing法的新型生物催化剂卤醇脱卤酶的设计与改造研究（编号20872014）；科技部政府间科技合作 项目名称：卤醇脱卤酶的蛋白质工程-基于精细化工品及药物中间体合成研究（编号3-10）；科技部政府间科技合作 项目名称：基于半理性设计策略进行卤醇脱卤酶HheA在合成手性功能砌块方面的改造研究（编号5-5）；教育部留学回国科研基金 项目名称：生物催化剂改造与挖掘技术及其应用研究（编号GGRYJJ08-3）；横向课题 项目名称：卤醇脱卤酶催化合成（R）-4-氰基-3-羟基-丁酸乙酯生物催化特性改造研究；横向课题 项目名称：卤醇脱卤酶催化特性改造研究
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| 研究成果：
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Juan Feng *, Qingqin Wu, Yu Zhou, Qizhou Hu, Ruiqin Fang, Lixia Tang. Interaction between flavin mononucleotide-containing Azoreductase and azo dyes. Spectroscopy letter, 2017, in press.
Ana Mikleu?evi?, Ines Primo?i?, Tomica Hrenar, Branka Salopek-Sondi, Lixia Tang, Maja Majeri? Elenkov. Azidolysis of epoxides catalysed by the halohydrin dehalogenase from Arthrobacter sp. AD2 and a mutant with enhanced enantioselectivity: an (S)-selective HHDH. Tetrahedron: Asymmetry (2016) 27, 930–935.
Wang, X., Han, S., Yang, Z., Tang, L.* (2015) Improvement of the thermostability and activity of halohydrin dehalogenase from Agrobacterium radiobacter AD1 by engineering C-Terminal amino acids. J Biotechnol., 212:92-98.（二区）
Tang, L.*, Liu, Y., Jiang, R., Zheng, Y., Zheng, K., Zheng, H. (2015) A high-throughput adrenaline test for the exploration of the catalytic potential of halohydrin dehalogenases in epoxide-ring opening reactions. Biotechnol. Appl. Biochem. 62:451-457.
Wang X., Lin H., Zheng Y., Feng J., Yang Z., Tang L. *(2015) MDC-Analyzer-facilitated combinatorial strategy for improving the activity and stability of halohydrin dehalogenase from Agrobacterium radiobacter AD1.J. Biotechnol. 206:1-7.（二区）
Mikleu?evic′, A., Hamer?ak, Z., Salopek-Sondi, B., Tang, L. Janssen, D.B., Majeri? -Elenkov, M. (2015) Oxazolidinone synthesis through halohydrin dehalogenase catalyzed dynamic kinetic resolution. Adv. Synth. Catal. 357:1709-1714. （二区）
Guo, C., Chen, Y., Zheng, Y., Zhang, W., Tao, Y., Feng, J, Tang, L.*(2015) Exploring the enantioselective mechanism of halohydrin dehalogenase from Agrobacterium radiobacter AD1 by iterative saturation mutagenesis. Appl. Environ. Microbiol. 2015 81(8):2919-26.（三区：IF3.952）
Schallmey, M., Jekel, P., Tang, L., Majeri? Elenkov, M., H?ffken, H.W., Hauer, B., Janssen, D.B. (2015) A single point mutation enhances hydroxynitrile synthesis by halohydrin dehalogenase. Enzyme Microb. Technol. 70:50-57.
Wang, X., Zheng, Z., Zheng, H., Nie, H., Yang, Z., and Tang, L.* (2014) DC-Analyzer-facilitated combinatorial strategy for rapid directed evolution of functional enzymes with multiple mutagenesis sites. J. Biotechnol. 192:102-107。（二区）
Tang, L.?, Wang, X., Ru, B., Sun, H., Huang, J., and Gao, H. (2014) MDC-Analyzer: a novel degenerate primer design tool for the construction of intelligent mutagenesis libraries with contiguous sites. BioTechniques 56:301-310. （二区）
Tang, L*., Zheng, K., Liu, Y., Zheng, Z., Wang, H., Song, C., Zhou, H. (2013) Exploring the potential of megaprimer PCR in conjunction with orthogonal array design for mutagenesis library. Biotechnol. Appl. Biochem. 60:190-195.
Bai, Y., Feng, J., Ma, H., Lin, J., Han, S., and Tang, L. (2013) Role of the CPC sequence in the antioxidant activity of GcGAST protein in E.coli. Protein J. 32: 48-57.
Majeri? Elenkov, M., Primo?i?, I., Hrenar, T., Smolko, A., Dokli, I., Salopek-Sondi, B., Tang, L. (2012) Catalytic activity of halohydrin dehalogenases towards spiroepoxides Org. Biomol. Chem. 10:5063-5072.（三区：IF 3.487）
Tang, L*., Gao, H., Zhu, X., Wang, X., Zhou, M. and Jiang, R. (2012) Construction of ‘small-intelligent’ focused mutagenesis libraries using well-designed combinatorial degenerate primers. BioTechniques 52:149-158. （二区）
Tang, L*., Zhu, X., Zheng, H., Jiang, R. and Majeri? Elenkov, M. (2012) Key residues for controlling enantioselectivity of halohydrin dehalogenase from Arthrobacter sp. AD2 revealed by structure-guided directed evolution. Appl. Environ. Microb.78:2631-2637. （三区：IF3.952）
Tang, L.*., Jiang, R., Zheng, K. and Zhu, X. (2011) Enhancing the recombinant protein expression of halohydrin dehalogenase HheA in Escherichia coli by applying a codon optimization strategy. Enzyme Microb.Technol., 49: 395-401.
Tang, L., Li, L., and Wang, X. (2010) A high-throughput colorimetric assay for screening halohydrin dehalogenase saturation mutagenesis libraries. J. Biotechnol. J. Biotechnol. 147, 164-168. （二区）
. Majeric′-Elenkov, M., Tang, L., Meetsma, A., Hauer, B. and Janssen, D.B. (2008) Formation of enantiopure 5-substituted oxazolidinones through enzyme-catalysed kinetic resolution of epoxides. Organic Letters 10, 2417-2420.
Majeric′-Elenkov, M., Wolfgang Hoeffken, H., Tang, L., Hauer, B. and Janssen, D. B. (2007) Enzyme-catalyzed nucleophilic ring opening of epoxides for the preparation of enantiopure tertiary alcohols. Adv. Synth. Catal. 349, 2279–2285.
Majeric′-Elenkov, M., Tang, L., Hauer, B. and Janssen, D.B. (2006) Sequential kinetic resolution catalyzed by halohydrin dehalogenase. Organic Letters 8, 4227-4229.
de Jong, R. M. Kalk, K. H. Tang, L., Janssen, D.B. and Dijkstra, B.M. (2006) The X-Ray Structure of the haloalcohol dehalogenase HheA from Arthrobacter sp. strain AD2: Insight into enantioselectivity and halide binding in the haloalcohol dehalogenase family. J. Bacteriology, 188, 4051-4056.
Tang, L., Torres Pazmi?o, D.E. Fraaije, M.W., and Janssen D.B. (2005) Improved catalytic properties of halohydrin dehalogenase by modification of the halide-binding site. Biochemistry 44, 6609-6618.
Hasnaoui, G., Lutje Spelberg, J. H., de Vries, E., Tang, L., Hauer, B., and Janssen, D. B. (2005) Nitrite-mediated hydrolysis of epoxides catalyzed by halohydrin dehalogenase from Agrobaterium radiobacter AD1: a new tool for the kinetic resolution of epoxides. Tetrahedron: Asymmetry 16, 1685-1692.
de Jong, R.M., Tiesinga, J.J.W., Villa, A., Tang, L., Janssen, D.B., and Dijkstra, B.W. (2005) Structural basis for the enantioselectivity of epoxide ring opening reactions catalyzed by haloalcohol dehalogenase HheC. J. AM. Chem. Soc. 127, 13338-13343.
Lutje Spelberg, J.H., Tang, L., Kellogg, R.M., and Janssen, D.B. (2004) Enzymatic dynamic resolution of epihalohydrins. Tetrahedron: Asymmetry 15, 1095-1102.
Tang, L., van Merode A.E.J., Lutje Spelberg J.H., Fraaije M.W., and Janssen D.B. (2003) Steady-state kinetics and tryptophan fluorescence properties of halohydrin dehalogenase from Agrobacterium radiobacter :roles of W139 and W249 in the active site and halide-induced conformational change. Biochemistry 42, 14057-14065.
de Jong, R.M., Tiesinga, J.J.W., Rozeboom, H.J., Kalk, K.H., Tang, L., Janssen, D.B., and Dijkstra, B.W. (2003) Structure and mechanism of a bacterial haloalcohol dehalogenase: a new variation of the short-chain dehygrogenase/reductase fold without an NAD(P)H binding site. EMBO J. 22, 4933-4944.
Tang, L., Lutje Spelberg, J.H., Fraaije, M.W., and Janssen, D.B. (2003) Kinetic mechanism and enantioselectivity of halohydrin dehalogenase from Agrobacterium radiobacter. Biochemistry 42, 5378-5386.
Tang L., Van Hylckama Vlieg, J.E.T., Lutje Spelberg, J.H., Fraaije, M.W., and Janssen, D.B. (2002) Improved stability of halohydrin dehalogenase from Agrobacterium radiobacter AD1 by replacement of cysteine residues. Enzyme Microb.Technol. 30, 251-258.
Lutje Spelberg, J.H., Tang, L., van Gelder, M., Kellogg, R.M., and Janssen, D.B. (2002) Exploration of the biocatalytic potential of a halohydrin dehalogenase using chromogenic substrates. Tetrahedron: Asymmetry 13, 1083-1089.
de Jong, R.M., Rozeboom, H.J., Kalk, K.H., Tang, L., Janssen, D.B., and Dijkstra, B.W. (2002) Crystallization and preliminary X-ray analusis of an enantioselective halohydrin dehalogenase from Agrobacterium radiobacter AD1. Acta Crystallographica D58, 176-178.
Van Hylckama Vlieg, J.E.T., Tang, L., Lutje Spelberg, J.H., Smilda, T., Poelarends, G.J., Bosma, T., van Merode, A.E.J., Fraaije, M.W., and Janssen, D.B. (2001) Halohydrin dehalogenases are structurally and mechanistically closely related to short-chain dehydrogenases/reductases. J. Bacteriol. 183, 5058-5066.
Lutje Spelberg, J.H., van Hylckama Vlieg, J.E.T., Tang, L., Janssen, D.B., and Kellogg, R.M. (2001) Highly enantioselective and regioselective biocatalytic azidolysis of aromatic epoxides. Organic Letters 3, 41-43.
Tang, L., Zhang, Y., Qian, Z., and Shen, X. (2000) The mechanism of Fe(2+)-initiated lipid peroxidation in liposomes: the dual function of ferrous ions, the roles of the pre-existing lipid peroxides and the lipid peroxyl radical. Biochem. J. 352, 27-36.
Tang, L., Yang, J., and Shen, X. (1997) Effects of additional iron-chelator on Fe2+ initiated lipid peroxidation: Evidence to support the Fe2+-Fe3+ complex as the initiator. J. Inorg.Biochem. 68, 265-272.
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| 专业研究方向：
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| 专业名称 |
研究方向 |
招生类别 |
| 083100生物医学工程 |
02生物学，08生物化学与分子生物学 |
博士学术学位 |
| 071000生物学 |
01生物化学与分子生物学 |
硕士学术学位 |
| 083100生物医学工程 |
08电子科技大学-加拿大麦吉尔大学生物医学工程-神经科学双硕士教育项目 |
硕士学术学位 |
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